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KMID : 0545120190290050758
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Journal of Microbiology and Biotechnology
2019 Volume.29 No. 5 p.758 ~ p.764
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Metabolic Engineering of Saccharomyces cerevisiae to Improve Glucan Biosynthesis
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Zhou Xing
He Jing
Wang Lingling
Wang Yang
Du Guocheng
Kang Zhen
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Abstract
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β-Glucan is a chief structural polymer in the cell wall of yeast. β-Glucan has attracted intensive attention because of its wide applications in health protection and cosmetic areas. In the present study, the β-glucan biosynthesis pathway in S. Cerevisiae was engineered to enhance β-glucan accumulation. A newly identified bacterial β-1, 6-glucan synthase GsmA from Mycoplasma agalactiae was expressed, and increased β-glucan content by 43%. In addition, other pathway enzymes were investigated to direct more metabolic flux towards the building of β-glucan chains. We found that overexpression of Pgm2 (phosphoglucomutase) and Rho1 (a GTPase for activating glucan synthesis) significantly increased β-glucan accumulation. After further optimization of culture conditions, the β-glucan content was increased by 53.1%. This study provides a new approach to enhance β-glucan biosynthesis in Saccharomyces cerevisiae.
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KEYWORD
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Saccharomyces cerevisiae, β-glucan, synthase, enzyme overexpression, cell resistance
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